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CSC8309 -- Gene Expression and Proteomics

We are going to investigate one time point, namely 12 hours after infection. There are three replicates for each.

act Load the affy data into the R environment using the following code.
### Load the appropriate library for reading affy data, and inspect the data.
library( 'affy' )
soy.ab <- ReadAffy( 'geo_data/GSM209576.CEL.gz',
                   'geo_data/GSM209585.CEL.gz',
                   'geo_data/GSM209594.CEL.gz',
                   'geo_data/GSM209577.CEL.gz',
                   'geo_data/GSM209586.CEL.gz',
                   'geo_data/GSM209595.CEL.gz',

                   ## we have gz files which R can read in.
                   compress=TRUE)



## Inspect the loaded data. This will make a network connection first time
## around and can be slow.
soy.ab

## Check out the names of the samples.
sampleNames( soy.ab )



## as the current sample names refer to the original files, we change this for
## something more, er, easy to remember.
new.sampleNames <- c('hr.a3.12','hr.b3.12','hr.c3.12',
                     'ts.a4.12','ts.b4.12','ts.c4.12')
sampleNames(soy.ab) <- new.sampleNames

## and check that it has worked
sampleNames( soy.ab )



(Complete File)(Rout)
quest
  1. How have I discovered the file names for the 12hr replicates? Read the GEO entry and make sure you understand.
  2. The new sample names are said to be "easy to remember". From where do the sample names come?

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