## we need to load some libraries and do some preparation on the initial data ## sets. the affy library is a set of standard analysis routines. while ALLMLL ## contains the data reported at Mary E. Ross, Xiaodong Zhou, Guangchun Song, ## Sheila A. Shurtleff, Kevin Girtman, W. Kent Williams, Hsi-Che Liu, Rami ## Mahfouz, Susana C. Raimondi, Noel Lenny, Anami Patel, and James R. Downing ## (2003) Classification of pediatric acute lymphoblastic leukemia by gene ## expression profiling Blood 102: 2951-2959 library( "affy" ) library( "ALLMLL" ) data( MLL.B ) Data <- MLL.B[, c(2,1,3:5,14,6,13)] sampleNames(Data) <- letters[1:8] ## these stats are some simple values that can be indicative of problems. ## simpleaffy calculates them all for you library( "simpleaffy" ) Data.qc <- qc(Data) ## this is average background -- they should all be about the same, f isn't avbg(Data.qc) ## scale factors, should be within 3x each other. f and g look bad sfs(Data.qc) ## are we missing lots of samples percent.present(Data.qc) ## 3/5 ratios... ratios(Data.qc)[,1:2]