A BLAST exercise

Now that you have an idea of how to do a BLAST search we’ll run through an example of our own.

Here is a mystery protein sequence that has been isolated by sequencing a clone from a library in your lab. Use BLAST with the non-redundant database to see if you can make a guess about its identity.

MRKMSSEEFYLFKNISSVGPWDGPQYHIAPVWAFYLQAAFMGTVFL

IFFPLNAMVLVATLRKLRQPLNYILVNVSFGGFLLCIFSVFPVFVA

SCNGYFVFGRHYCALEGFLGTVAGLVTWWSLAFLAFERYIVICKPF

GNFRFSSKHALTVVLATWTIGIGVSIPPFFGWWWFIPEGSCGPDDW

YTVGTKYRSESYTWFLFIFCFIVPLSLICFSYTQLLRALKARIRSF

FPYFLICQGYLQLYLSLQFQACIMKMVCGKAMTDESDTCSSVKTEV

STVSSTQVGPN

Tips on interpreting BLAST output from the Australian National Genomic Information Service

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